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Figure 1 | Microbiome

Figure 1

From: CloVR-ITS: Automated internal transcribed spacer amplicon sequence analysis pipeline for the characterization of fungal microbiota

Figure 1

Flowchart of the CloVR-ITS protocol. Preprocessing of sequences includes filtering for quality and length, clustering to remove redundant sequence information as well as de novo chimera detection. After preprocessing, sequences are clustered into genus-level operational taxonomic units and assigned to a taxonomic lineage using BLASTN with a custom database of reference fungal species (clovr-itsdb v1.0). The pipeline also performs alpha- and beta-diversity analyses including rarefaction curves, computation of ecological estimators, differential abundance detection, and unsupervised clustering of samples according to taxonomic profiles.

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