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Table 2 Primers designed for this study are illustrated

From: A gene-targeted approach to investigate the intestinal butyrate-producing bacterialcommunity

Functional genes - pyro-sequencing

buk_1F

atcaaYccDggWtcWacWtcWac

buk_1R

acHgcYttYtgRtttaaWgcatg

buk_2F

atWaatccWggttcWacWtcWacMaa

buk_2R

tgcYttYtggttgagygc

buk_3F

atMaaTccWggBtcKacMtcaact

buk_3R

gccttctgRttMagKgcatg

but_1F

cagctIggYatYggIgS

but_1R

aaRtccaIYtgIccVcc

but_2F

ggWatWggMgsYatgcc

but_2R

aaRtcaaSctgKccDc

but_3F

gHatYggIgStatgcc

but_3R

aagtcWaaYtgwccRcc

Functional genes - quantitative PCR

G_buk_F

tgctgtWgttggWagaggYgga

G_buk_R

gcaacIgcYttttgatttaatgcatgg

G_Acida_F

cgcagaagaacattgacaagg

G_Acida_R

atggcagggttattgtctacataatc

G_Fprsn_F

gacaagggccgtcaggtcta

G_Fprsn_R

ggacaggcagatRaagctcttgc

G_RosEub_F

tcaaatcMggIgactgggtWga

G_Ros_R

tcgataccggacatatgccaKgag

G_Eub_R

tcataaccgcccatatgccatgag

16S genes - quantitative PCR

Cbuty_F

tactctgtaatggaggaagccact

Cbuty_R

ggtacaatgagatgcaacctcgc

FPR-2F a

ggaggaagaaggtcttcgg

Fprau645R a

Aattccgcctacctctgcact

Rrec630F a

cgKactagagtgtcggagg

Erec870R a

agtttYattcttgcgaacg

RrecRi630F a

gtcatctagagtgtcggagg

1132F b

atggYtgtcgtcagctcgtg

1108R b

Gggttgcgctcgttgc

  1. Degenerate bases are shown in capital letters. The following sequences aretargeted: buk_F/R - butyrate kinase (buk) genes; but_F/R -butyryl-CoA:acetate CoA-transferase (but) genes; G_buk_F/R -buk genes of Clostridium acetobutylicum, C.butyricum, C. perfringens; G_Acida - but genes ofAcidaminococcus sp.; G_Fprsn - but genes ofFaecalibacterium prausnitzii; G_RosEub, G_Ros_R, G_Eub_R -but genes of Eubacterium rectale and Roseburiasp; Cbuty_F/R -16S genes of C. butyricum; FPR-2 F, Fprau645R- 16S genes of F. prausnitzii; Rrec630F, RrecRi630F, Erec870R - 16Sgenes of E. rectale and Roseburia sp.; 1132 F, 1108R- universal for 16S. a Primers described in Ramirez-Farias etal.[18]; bPrimers described in Leigh et al.[19]. For more details on targeted sequences seeAdditional file 1: Table S1 and S2.