Skip to main content
Figure 2 | Microbiome

Figure 2

From: MaxBin: an automated binning method to recover individual genomes from metagenomes using an expectation-maximization algorithm

Figure 2

Binning performance estimated from two 10-genome simulated datasets. Only scaffolds longer than 1,000 bps were used. Each bar represents an individual genome; blue, red, and green parts indicate correctly assigned, mis-assigned, and unclassified scaffolds, respectively. In other words, the presence of red bars indicates that part of the genome has been incorrectly assigned to bins belonging to other species, and green bars are genome parts that are unclassified. These do not count scaffolds shorter than 1,000 bps since these scaffolds will be discarded before applying the expectation-maximization algorithm and do not reflect the performance of MaxBin. Yellow lines represent the relative abundance ratios of the corresponding species. The Y-axis at the left and right side indicate binned genome sequences in million bps and genome abundances in relative abundance ratio (%). The species names of the genomes were indicated in Additional file 1: Table S1. (A) 80X simulation. (B) 20X simulation. An entire red bar, such as the fourth bin in the 20X simulation, indicates that scaffolds of this species have been incorrectly assigned to other bins. Genomes with much shorter bars indicate that these genomes were assembled poorly and hence only a small proportion of scaffolds are longer than 1,000 bps and show up in the figures.

Back to article page