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Table 1 Pacific Biosciences circular consensus recruiting to each genome and genome coverage

From: Caught in the middle with multiple displacement amplification: the myth of pooling for avoiding multiple displacement amplification bias in a metagenome

   

Control

MDA5

MDA1

Genome*

%GC

Predicted read abundance†(%)

CCS reads recruited

Read abundance (%)

Coverage (±SD)

CCS reads recruited

Read abundance (%)

Coverage (±SD)

CCS reads recruited

Read abundance (%)

Coverage (±SD)

Blue7-1

61.4

15.5

4,631

25.9

98.8 (19.5)

2,380

13.2

43.8 (19.4)

1,522

13.4

33.9 (13.9)

Fuitloop-1

61.8

31.1

7,165

40.1

132.1 (25.5)

8,341

46.4

140.5 (82.4)

5,419

47.8

111.4 (65.7)

Gumball-1

59.6

20.7

1,230

6.9

15.4 (6.1)

3,460

19.2

52.5 (25.1)

2,007

17.7

37.2 (17.9)

Porky-1

63.5

25.9

3,271

18.3

46.3 (7.3)

1,401

7.8

18.1 (12.2)

889

7.8

13.6 (8.8)

Wee-1

61.8

5.2

1,127

6.3

20.3 (5.6)

2,216

12.3

35.8 (22.1)

1,391

12.3

27.3 (15.3)

Gumball-2

59.6

20.8

495

5.4

6.2 (3.0)

1,261

6.5

18.1 (9.4)

1,613

6.5

24.0 (12.6)

Lambda-2

49.9

15.6

3,737

40.7

84.7 (12.0)

10,995

56.3

208.7 (107.1)

14,284

57.5

274.6 (130.7)

Porky-2

63.5

24.5

1,121

12.2

16.1 (3.7)

664

3.4

8.2 (6.5)

815

3.3

10.1 (7.3)

T7-2

48.4

12.8

1,050

11.4

29.8 (5.6)

3,920

20.1

90.2 (30.7)

5,029

20.2

115.7 (37.7)

VBP32-2

42.5

24.9

2,616

28.5

37.5 (8.9)

2,373

12.1

27.6 (15.9)

2,821

11.4

33.5 (17.2)

  1. *-1, -2 indicates data from community one and community two, respectively. †Predicted read abundances were recalculated to take into account the low recruitment of phage Athena, T4 and VBpm10. CCS, circular consensus sequencing; MDA, multiple displacement amplification.