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Figure 4 | Microbiome

Figure 4

From: 16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles

Figure 4

Normalization of PCR amplification by choosing PCR cycle number close to the Ct value. Throat swabs from healthy volunteers were extracted with Qiagen DNeasy (D) or MO BIO PowerSoil (M). DNA extracts were subjected to 16S gene TaqMan qPCR and subsequent PCR using a cycle number of 20, 25, or 30 based on individual sample's qPCR Ct value. PCR amplicons were quantified by PicoGreen dsDNA assay. DNA concentrations for DNA extracts (blue markers and axis) and PCR amplicons (red markers and axis) are shown in 16S gene copies/μl in the same scale. DNA sample name, 08S1M as an example, depicts the subject number (08), swab number (S1), and extraction method (M).

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