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Figure 5 | Microbiome

Figure 5

From: 16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles

Figure 5

Relative bacterial abundance determined by OTU from 454 pyrosequencing analysis. HM-278D and HM-279D were amplified by PCR for 20 cycles, respectively. Data for mock community DNA equal-molar mix used in HMP studies (HMP-MC) were from the HMP Data Analysis and Coordination Center (DACC) and NCBI. All data were analyzed using the QIIME-based pipeline, with classification of operational taxonomic units (OTUs) to bacterial genus level. Left panel, mock community DNA equal-molar mix. Right panel, mock community DNA staggered-molar mix. Projected, the relative bacterial abundance calculated based on DNA quantities used in making the HM-278D and HM-279D.

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