Structure and function of the healthy pre-adolescent pediatric gut microbiome

Microbiome

Table 1 Taxa contributing to the classification of child versus adult gut communities

Analysis type	Taxon identity (OTU or species)	Random forests importance score	Mean relative abundance (%)^a		White’s non-parametric t-test
Analysis type	Taxon identity (OTU or species)	Random forests importance score	Child	Adult	p value	q value
16S	OTU_1555 Anaerovorax	6.77	0.23	0.16	9.99E−04	0.028
16S	OTU_1412 Bifidobacterium	6.07	0.34	0.01	9.99E−04	0.028
16S	OTU_1015 Faecalibacterium	5.28	8.26	3.52	9.99E−04	0.028
16S	OTU_411 Collinsella	4.79	0.20	0.01	9.99E−04	0.028
16S	OTU_2162 Lachnospiraceae Incertae Sedis	4.74	0.93	0.28	9.99E−04	0.028
16S	OTU_2956 Porphyromonadaceae	4.69	0.01	0.08	9.99E−04	0.028
16S	OTU_3384 Bacteroides	4.24	0.08	0.20	9.99E−04	0.028
16S	OTU_4352 Lachnospiraceae	4.04	3.52	1.65	1.99E−03	0.053
16S	OTU_1928 Ruminococcaceae	3.60	0.38	0.08	9.99E−04	0.028
16S	OTU_987 Ruminococcaceae	3.53	0.57	0.09	9.99E−04	0.028
WGS	Bifidobacterium longum	6.01	6.54	0.24	9.99E−04	0.018
WGS	Eggerthella lenta	5.44	0.06	0.01	1.00	1.000
WGS	Porphyromonas asaccharolytica	5.13	6.30E−03	0.03	4.92E−01	0.888
WGS	Clostridium asparagiforme	5.13	1.24E−02	6.67E−03	1.00	1.000
WGS	Streptococcus sanguinis	4.64	0.01	4.09E−04	1.00	1.000
WGS	Faecalibacterium prausnitzii	4.63	7.39	1.31	9.99E−04	0.018
WGS	Faecalibacterium cf	4.56	4.47	0.68	9.99E−04	0.018
WGS	Bifidobacterium catenulatum	4.37	0.33	0.02	8.40E−03	0.069
WGS	Gordonibacter pamelaeae	3.94	0.21	0.01	6.30E−02	0.313
WGS	Granulicatella adiacens	3.76	1.00E−03	6.36E−06	1.00	1.000

Taxa were identified as a function of their Random Forests permutation importance values. OTU identities were generated using the RDP Classifier with a confidence threshold of 50 %, and species identities were generated from the shotgun metagenomic libraries using MetaPhlAn. Differences in the relative abundance of each taxon were evaluated using two-tailed White’s non-parametric t-test, and Storey’s false discovery rate estimator was used to correct for multiple testing corrections within each data set (i.e., 16S, WGS)
^a n = 37 children and 43 adults in the OTU-based analysis and 22 children and 22 adults in the WGS-based analysis

ISSN: 2049-2618