Table 5 Primers used for 16S rRNA gene amplification of gDNA extracted from stools
From: Characterizing the fecal microbiota of infants with botulism
Fusion PCR Primer | Sequencing adapters | Library key | 16S rRNA Gene target region | Target name |
|---|---|---|---|---|
A forward | 5’-CCATCTCATCCCTGCGTGTCTCCGAC | TCAG | CCTACGGGAGGCAGCAG-3’ | P1 |
trP1 forward | 5’-CCTCTCTATGGGCAGTCGGTGAT | – | CCTACGGGAGGCAGCAG-3’ | P1 |
A reverse | 5’-CCATCTCATCCCTGCGTGTCTCCGAC | TCAG | ATTACCGCGGCTGCT-3’ | P2 |
trP1 reverse | 5’-CCTCTCTATGGGCAGTCGGTGAT | – | ATTACCGCGGCTGCT-3’ | P2 |