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Table 2 OTUs with differential abundances over long term storage at −80 °C

From: Latitude in sample handling and storage for infant faecal microbiota studies: the elephant in the room?

 

Mean reads (% of group total) at:

    

Significant after a MHC?

OTU

2 months

24 months

Exponentiated coefficient

2.5 % CI

97.5 % CI

P value

Lactobacillus1

3.306

5.596

1.021

1.012

1.029

>0.001

Yes

Lactobacillus2

0.109

0.258

1.035

1.014

1.056

0.001

Yes

Bacilli

0.585

0.735

1.013

1.001

1.026

0.028

No

Enterobacteriaceae

0.683

0.218

0.948

0.934

0.962

>0.001

Yes

Gammaproteobacteria1

0.620

0.304

0.970

0.955

0.986

>0.001

Yes

Enterococcus2

0.207

0.396

1.031

1.014

1.048

>0.001

Yes

Staphylococcus1

0.247

0.189

0.975

0.956

0.994

0.009

No

Staphylococcus2

0.339

0.143

0.967

0.944

0.990

0.005

No

  1. The OTUs described shift significantly over the course of long term storage at −80 °C. Mean sequencing reads in the samples stored for 2 months and those stored for 24 months are shown. The exponentiated coefficients were provided by the GLMs and approximate the rate of change in relative abundance for each OTU per month of storage; 2.5 and 95 % CI indicate the 95 % confidence interval around the exponentiated coefficient. P values indicate the significance of the coefficient (prior to MHC for 24 tested OTUs).The final column indicates whether the result would still be significant after a Bonferroni correction