Rumen metagenome and metatranscriptome analyses of low methane yield sheep reveals a Sharpea-enriched microbiome characterised by lactic acid formation and utilisation

Table 1 Bacterial taxa (97 % sequence similarity) with taxonomy assigned to highest possible resolution, differing in mean relative abundance (%) between HMY and LMY animals measured at two time points. Significances are based on one-way ANOVA and Bonferroni corrected P values

Taxon: order/family/genus	Methane yield group	Amplicon			Metagenome			Spearman
Taxon: order/family/genus	Methane yield group	P	Low	High	P	Low	High	R	P
Clostridiales/Christensenellaceae	High	<0.01	0.05	0.42	NS	0.05	0.27	0.8	<0.01
Clostridiales/Lachnospiraceae/Anaerostipes	High	<0.01	0.12	1.2	NS	0.13	1.02	0.58	<0.01
Verrucomicrobia/Verruco-5/WCHB1/RFP12	High	<0.01	0.02	0.17	<0.01	0.03	0.16	0.67	<0.01
Bacteroidales/BS11	High	NS	0.03	0.35	<0.05	0.01	0.1	0.6	<0.01
Erysipelotrichales/Erysipelotrichaceae/Sharpea	Low	<0.05	6.43	0.49	<0.05	6.3	0.58	−0.52	<0.01
Coriobacteriales/Coriobacteriaceae/Collinsella aerofaciens	Low	<0.01	0.42	0.02	<0.05	0.62	0.01	−0.6	<0.01
Clostridiales/Eubacteriaceae/Pseudoramibacter	Low	<0.05	0.19	0.09	NS	0.25	0.1	NA	NA
Clostridiales/Veillonellaceae/Megasphaera	Low	<0.05	1.02	0.06	NS	1.41	0.07	−0.54	<0.01
Erysipelotrichales/Erysipelotrichaceae/Sharpea azabuensis	Low	<0.05	7.47	0.55	<0.05	7.83	0.68	−0.7	<0.01

Taxa with significant difference (P ≤ 0.05) in either 16S rRNA gene amplicon or 16S rRNA gene metagenome sequence abundance are shown. Spearman’s Rank Correlation based on amplicon sequencing data is included where −0.5 ≤ R ≥ 0.5 and P ≤ 0.01
NS not significant, NA not applicable

ISSN: 2049-2618