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Fig. 1 | Microbiome

Fig. 1

From: Large-scale benchmarking reveals false discoveries and count transformation sensitivity in 16S rRNA gene amplicon data analysis methods used in microbiome studies

Fig. 1

Spike-in approach and analysis flowchart. Left, a theoretical sample where the count data for OTU A is multiplied by 3 before rescaling to original sequencing depth. Right, flowchart of the simulation study, yielding FPR and AUC for each method, dataset, and set of variables, as well as R 2 values for all combinations of normalization, transformation, and distance in the beta-diversity optimization

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