Fig. 4

Trichlorphon tolerance and degradation ability of CF-BD and its effects on the drug susceptibility and resistance of flies. a A comparison of the number of CF-BD colony forming units (mean ± SE) between the RS flies and those that were given streptomycin. b Toxicity regression analyses of the RS (red line) and streptomycin-fed RS (green line) fly responses to trichlorphon. c A comparison of CF-BD colony numbers (mean ± SE) between SS flies and CF-BD-fed SS flies. d Toxicity regression analyses of of SS (green line) and CF-BD-fed SS (red line) fly responses to trichlorphon. e Characteristics of bacteria that were cultivated on trichlorphon-enriched plates and normal plates. f Diameters of the bacterial colonies (mean ± SE) cultivated on trichlorphon-enriched plates and normal plates. g The gas chromatograph identification of trichlorphon in the filtrate of mineral media with or without CF-BD cultures. Differences in the trichlorphon concentrations were evaluated by comparing the peak areas (mean ± SE) that were identified by gas chromatography. h The gas chromatography mass spectrometry (GC-MS) identification of the trichlorphon degradation products of CF-BD (the degradation pathway is presented). The significant differences at the p < 0.01 level, as evaluated with independent-sample t tests, are indicated by two asterisks above the bars. ns indicates not significant. Tr trichlorphon-enriched plates, CK control plates