Fig. 1From: Novel soil-inhabiting clades fill gaps in the fungal tree of lifePrimer map indicating the construction of long 18S-ITS-28S rRNA gene sequences from 454 pyrosequencing-based ITS2 amplicons (in red) and Sanger sequencing of flanking conservative genes using taxon-specific reverse (Rev) and forward (Fwd) primers in combination with eukaryote primers (in blue). Primer information is given in Additional file 2: Table S1Back to article page