Fig. 5

The intestinal dysbiosis triggered by ATT is long-lasting and persists following cessation of treatment. a Community diversity in the naïve and post HRZ animal groups calculated from 16S sequences (W24–W32) using Chao1 (left) and Shannon (right) indices. Error bars indicate maximum and minimum values. b Principal coordinate (PC) analysis of unweighted (top) and weighted (bottom) UniFrac distances of the sequences from the two groups. Sizes of spheres depict the time of sample collection as described in Fig 1b. One sample each from W24, W28, and W32 time points of the naïve group was not included in the analysis since these three samples formed a separate independent cluster inconsistent with the other clusters. (For comparison, these samples are included, nevertheless, in Additional file 13: Figure S13). c Average relative abundance of bacterial families identified from the sequenced data of the naïve and post HRZ stool samples (W24–W32). The bacterial families are grouped under their respective phylum and class in the color key. d Heat map showing the average species level relative abundance. Data are filtered for overall relative variance >5 and clustered as described in Fig. 1c. e LEfSe comparisons showing the differentially abundant genera between the naïve and post HRZ groups (W24–W32). Taxa significantly enriched in naïve or post HRZ groups are depicted with blue or yellow bars, respectively. Data are filtered for p < 0.05 and LDA score >2. n = 4–5