Fig. 2From: More than just a gut feeling: constraint-based genome-scale metabolic models for predicting functions of human intestinal microbesSuggested cultivation strategy. The initial cultivation strategy of a microbe can be optimized by thorough analysis of its genome and isolation conditions. The genome contains information on metabolic pathways, as represented in GEMs, that inform on auxotrophies and suitable carbon, nitrogen, and sulphur sources. In addition, the genome annotation can reveal additional considerations such as antibiotic or bile resistance, or the ability to form spores. The isolation condition of a microbe, for example the human gut, provides information on suitable environmental conditions such as temperature, pH, and ion strengthBack to article page