Fig. 4

RNA transcripts of major metabolic processes in the sediments. Selected functional groups of RNA transcripts from the sediment after incubation in darkness for 21 days (RNA transcripts that were ≥100 FPKM in one of the treatments are shown). Functional groups were chosen based on statistical significance from edgeR analysis of the studied chemistry and 16S rRNA gene data. a Anoxic-to-oxic (n = 2, two left bars for each gene) and anoxic control (n = 2, two right bars for each gene). b Intermediate-to-oxic (n = 2, left bars) and intermediate-to-anoxic (n = 2, right bars). c Oxic-to-anoxic (n = 2, left bars) and oxic control (n = 1, right bar). Stars denote a fold change of at least 1.5 for the average of the two replicates compared to each replicate of the other shown treatment. All values show TMM normalized FPKM units. The colors denote relative abundance (% relative to column height) of taxonomic groups for the annotated RNA transcripts (i.e., derived from top hit organism in the UniprotKB/Swiss-Prot database)