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Table 1 Primers and annealing temperatures used for the detection of antibiotic resistance genes by PCR

From: Impact of intrapartum antimicrobial prophylaxis upon the intestinal microbiota and the prevalence of antibiotic resistance genes in vaginally delivered full-term neonates

Gene

Oligonucleotide

Annealing T°

Antibiotic group

Resistance mechanisms

Ref.

tet(W)

F-AAGCGGCAGTCACTTCCTTCCR-TCAAGTATCCCAGCGAAACC

60

Tetracyclines

Ribosomal Protection Protein

[49]

tet(M)

F-ACAGAAAGCTTATTATATAACR-TGGCGTGTCTATGATGTTCAC

55

Tetracyclines

Ribosomal Protection Protein

[49]

tet(O)

F-ACGGARAGTTTATTGTATACCR-TGGCGTATCTATAATGTTGAC

60

Tetracyclines

Ribosomal Protection Protein

[49]

tetA(B)

F-TTGGTTAGGGGCAAGTTTTGR-GTAATGGGCCAATAACACCG

55

Tetracyclines

Efflux pump

[63]

bla tem

F-TTTCGTGTCGCCCTTATTCCR-CCGGCTCCAGATTTATCAGC

60

Penicillins

β-lactamase

[63]

bla CTX-M

F-ATGTGCAGYACCAGTAARGTKATGGCR-GGGTRAARTARGTSACCAGAAYSAGCGG

60

Penicillins

β-lactamase

[50]

bla SHV

F-CACTCAAGGATGTATTGTGR-TTAGCGTTGCCAGTGCTCG

58

Penicillins

β-lactamase

[64]

mecA

F-GGGATCATAGCGTCATTATTCR-AACGATTGTGACACGATAGCC

56

Penicillins

Penicillin-binding protein 2a

[50]

aac(6″)-Ie-aph(2″)

F-CCAAGAGCAATAAGGGCATACCR-CACACTATCATAACCATCACCG

55

Aminoglycosides

Bifunctional acetyltransferase phosphotransferase

[64]

strA

F-CTTGGTGATAACGGCAATTCR-CCAATCGCAGATAGAAGGC

65

Aminoglycosides

Phosphotransferase

[63]

cmlA1

F-CACCAATCATGACCAAGR-GGCATCACTCGGCATGGACATG

60

Chloramphenicol

Efflux pump

[63]