Fig. 3

Distribution of enzymes in metabolic pathways for different sample-wise comparisons. For each of the 36 KEGG metabolic pathways listed, the number (a) and average expression fold change (b) of significantly differentially expressed enzymes (significant enzymes) are indicated for each of the four pairwise comparisons. Detailed q values are provided in Additional file 6: Table S5. Significant enzymes are defined as enzymes to which at least one significantly differentially expressed transcript is mapped (see “Methods”). Numbers in brackets after pathway name indicate the total number of enzymes associated with each pathway. Twenty-one of the 36 pathways belong to one of the four KEGG-defined super families: (1) amino acid metabolism, (2) energy metabolism, (3) carbohydrate metabolism, and (4) nucleotide metabolism. The ratio of expressed enzymes indicates the proportion of enzymes associated with that pathway that was expressed in at least one of the samples. Asterisks indicate pathways that were statistically significantly enriched in significant enzymes (p value < 0.05) for each genotype/diet comparison, e.g., Geno-HF (red), Geno-LF (blue), Diet-Plin2 (green), and Diet-WT (purple)