Enrichment allows identification of diverse, rare elements in metagenomic resistome-virulome sequencing

Microbiome

Table 1 Diversity statistics, median (range). Medians with different letters were significantly different (Tukey P < 0.05). Medians without letters were not subjected to pairwise comparison because the omnibus ANOVA test was not significant (P < 0.05)

Assay	Gene-level richness	Gene-level Simpson’s diversity	Gene-level Simpson’s equitability
Metagenome	277 (23–685)^ab	14.5 (7.8–105.4)	0.14 (0.01–0.47)^abc
Metagenome-UMI	366 (34–575)^ab	23.8 (3.7–149.8)	0.11 (0.01–0.36)^ab
Resistome	1074 (595–1330)^cd	59.0 (9.2–142.5)	0.06 (0.01–0.11)^acd
Resistome-UMI	766 (493–1265)^cd	43.3 (16.5–144.4)	0.06 (0.03–0.12)^cd

ISSN: 2049-2618