Fig. 2
From: Discovery of the fourth mobile sulfonamide resistance gene
Schematic diagram of the different arrangements of sul4 recovered by amplicon sequencing of integron datasets: a RSPETL, Hyderabad, India. The read begins with an attI site, as the integron-integrase gene was not amplified by the primers. Alignment of 1.10 Kbp of the gene cassettes to the read in b RSPune, Pune and RSPETL, Hyderabad, India. The first gene cassette is a hypothetical protein (WP_019224580.1). Primers used to further confirm the context found in RSPune and RSPETL are indicated by arrows. The rest of the arrangements are contigs that resulted from assembling different shotgun metagenomics datasets. c mgm4622354.3 (MG-RAST ID) collected from Kolkata, India. d mgm4510219.3 collected from Malaysia. e mgm4709385.3 collected from Sheffield, UK. f ERR1414268 (EBI ID) collected from Käppala sewage treetment plant, Sweden. Downstream of ISCR20 are hypothetical proteins (WP_076836759.1 and WP_011927925.1) g mgm4537907.3 collected from Hong Kong and h mgm4714564.3 collected from Beijing, China. Due to the lower read coverage, contigs covering the entire sul4 gene were not recovered in the f–h metagenomes