Fig. 3

Screening for deletion mutants unable to induce the growth of Faecalibacterium sp. KLE1255. a A selection of 283 strains that were compiled from E. coli small-, medium-, and large-scale deletion libraries to cover all non-essential genes of the E. coli genome were screened for induction of KLE1255 via co-culture assay. b Eight strains were identified as being unable to induce growth of KLE1255, harboring deletions in various regions of the genome. c One strain, E. coli OCL67, had a single large deletion consisting of 16 genes, including 6 in menaquinone biosynthesis. menE = 2-succinylbenzoate--CoA ligase; menC = o-succinylbenzoate synthase; menB = 1,4-dihydroxy-2-naphthoyl-CoA synthase; menH = 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase; menD = 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase; menF = isochorismate synthase; elaB = putative membrane-anchored DUF883 family ribosome-binding protein; elaA = putative N-acetyltransferase; rbn = ribonuclease BN; elaD = deubiquitinase; yfbK = putative lipoprotein; yfbL = putative membrane-associated peptidase; yfbM = DUF1877 family protein; yfbN = uncharacterized protein; yfbO = uncharacterized protein; yfbP = uncharacterized protein. d Wild type induced strong growth in KLE1255, while e OCL67 did not. Similarly, f WT E. coli also induces Faecalibacterium prausnitzii ATCC 27766