Fig. 5

Specific amino acid auxotrophies and vitamin auxotrophies present in high-quality GBs. The auxotrophy of specific compound was confirmed if more than one gene was absent in the synthetic pathway, or overall transcriptional level of pathway was low compared to genomes with complete pathway in which only one gene was absent. Aerobic species are covered in gray. Amino acids are in depicted in blue and vitamins in brown. Amino acids have been ranked according to biosynthetic cost (arrow). A colored square denotes that a species can synthesize an amino acid. The intensity of each color (based on the scale) represents the relative expression of the synthesis pathways, with darker/more intense color indicating higher expression. Auxotrophies are marked by a red cross. A black line next to the box represents the presence of a transporter. A transport system is not required for vitamins B2 and B3