Fig. 2

Evaluation of GCN prediction tools on genomes with known GCNs. Accuracy of GCN predictions by CopyRighter (a; [7]), PICRUSt (b; [6]), and PAPRICA (c; [8]) for sequenced genomes, as a function of the genome’s NSTD. NSTDs were calculated separately for each tool, based on the set of genomes used to calibrate the tool by its authors. Accuracy was measured in terms of the coefficient of determination, i.e. the fraction of variance in true GCNs explained by each tool (R²). Genomes were binned into equally sized NSTD intervals (i.e., 0–10%, 10–20% etc.), and the R² was calculated separately for genomes in each bin (one plotted point per bin). Only bins with at least 10 genomes are shown