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Fig. 3 | Microbiome

Fig. 3

From: Correcting for 16S rRNA gene copy numbers in microbiome surveys remains an unsolved problem

Fig. 3

Comparisons of 16S GCN predictions between tools across Greengenes. a 16S GCNs predicted by CopyRighter (vertical axis; [7]) and PICRUSt (horizontal axis; [6]) across OTUs (99% similarity) in the Greengenes 16S rRNA reference database (release May 2013; [20]). One point per OTU. b Comparison of predicted 16S GCNs by PICRUSt and PAPRICA, similarly to (a). c Comparison of predicted 16S GCNs by CopyRighter and PAPRICA, similarly to (a). Diagonal lines are shown for reference. Fractions of explained variance (R2, X-axis explaining Y-axis) and the number of considered OTUs (n) are written in each figure. d–f Fractions of explained variance (R2) as a function of an OTU’s NSTD, for each compared pair of tools in a–c. OTUs were binned into equally sized NSTD intervals (i.e., 0–5%, 5–10% etc.), and the R2 was calculated separately for OTUs in each bin (one plotted point per bin). Only bins with at least 10 OTUs are shown

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