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Fig. 3 | Microbiome

Fig. 3

From: Predominant gut Lactobacillus murinus strain mediates anti-inflammaging effects in calorie-restricted mice

Fig. 3

Calorie restriction rapidly creates a Lactobacillus-predominated gut microbial community in mice in 14 days. a PCoA based on Bray-Curtis distance. b Alteration of the gut microbiota structure along the first principal component (PC1) of the PCoA based on Bray-Curtis distance. Data were plotted as the mean ± s.e.m. Mann-Whitney U test (two-tailed) was used to analyse variation between the NC and CR groups at the same time point. **P < 0.01 and ***P < 0.001 vs the NC group at the same time point. c Co-abundance network illustrating the interactions among the 50 key OTUs in the CR group. The 50 OTUs were grouped into eight co-abundance groups (CAGs) by permutational multivariate analysis of variance (PerMANOVA) test when P < 0.001. Different colours and shapes of nodes represent different CAGs and phyla, respectively. The line connecting two nodes represents the correlation between the connected nodes, with the colour saturation and line width indicating the correlation magnitude: red represents a positive correlation, and blue represents a negative correlation. Only lines corresponding to correlations with a magnitude greater than 0.5 were drawn. The plots show the relative abundance of each CAG in the CR group in the first 14 days on a daily basis. Data in the plots represent the total relative abundance of all OTUs in each CAG from each sample of the CR group and are reported as the mean ± s.e.m. N in the plots indicates the days after calorie restriction when a specific CAG began to change, as determined by an absolute value of its logarithmic (base 2)-fold change in relative abundance greater than 1 (|log2-fold change| > 1). Kruskal-Wallis test followed by Dunn’s multiple comparison test was used to analyse variation relative to day 0. *P < 0.05, **P < 0.01 and ***P < 0.001 vs day 0. n = 10 for both NC and CR groups at all time points except n = 9 for the NC group at day 10 and day 11 and n = 9 for the CR group at day 2

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