Fig. 5

Identification and genomic characterization of the two strains of L. murinus enriched by calorie restriction. a Phylogenetic relationships of the Lactobacillus isolates with their relatives based on 16S rRNA gene sequences. The tree was constructed using the Neighbour-Joining method in MEGA7, and Lactococcus lactis NCDO 607T was used as an outgroup. The bar indicates sequence divergence. b Functional categories in the clusters of orthologous groups (COG) analysis of L. murinus CR141 and CR147. The middle circle represents the number of COG families shared by the two strains in each category. c Linear genomic comparison of the chromosomes of L. murinus CR141 and CR147. Both sequences are read left to right from the predicted replication origin. Homologous regions within the two genomes identified by BLASTN are indicated by red (same orientation) and blue (reverse orientation) bars. Prophage-related regions (red boxes), GI (genomic island)-like regions (purple boxes) and transposases (green boxes) identified by VRprofile are indicated. The strain-specific CDSs with assigned functions are also labelled