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Table 1 Effect of the level of P, E and F synthetic spikes. (pg DNA) added to Bawburgh and Wytham soil (1 g)

From: Absolute quantitation of microbiota abundance in environmental samples

Synthetic spike level

Amount relative to level 8 (%)

P synthetic spike (pg)

Copies of 16S rRNA added

E synthetic spike (pg)

Copies of 18S rRNA added

F synthetic spike (pg)

Copies of ITS added

1

1

97

3.5E + 07

57

2.1E + 07

32

1.2E + 07

2

2

193

7.1E + 07

113

4.3E + 07

64

2.4E + 07

3

5

483

1.8E + 08

283

1.1E + 08

161

5.9E + 07

4

10

966

3.5E + 08

566

2.1E + 08

321

1.2E + 08

5

15

1449

5.3E + 08

849

3.2E + 08

482

1.8E + 08

6

20

1931

7.1E + 08

1132

4.3E + 08

643

2.4E + 08

7

50

4829

1.8E + 09

2831

1.1E + 09

1607

5.9E + 08

8

100

9657

3.5E + 09

5661

2.1E + 09

3213

1.2E + 09

Spike (1 ng) contains

3.7E + 08

 

3.8E + 08

 

3.7E + 08