From: Absolute quantitation of microbiota abundance in environmental samples
Synthetic spike level | Amount relative to level 8 (%) | P synthetic spike (pg) | Copies of 16S rRNA added | E synthetic spike (pg) | Copies of 18S rRNA added | F synthetic spike (pg) | Copies of ITS added |
---|---|---|---|---|---|---|---|
1 | 1 | 97 | 3.5E + 07 | 57 | 2.1E + 07 | 32 | 1.2E + 07 |
2 | 2 | 193 | 7.1E + 07 | 113 | 4.3E + 07 | 64 | 2.4E + 07 |
3 | 5 | 483 | 1.8E + 08 | 283 | 1.1E + 08 | 161 | 5.9E + 07 |
4 | 10 | 966 | 3.5E + 08 | 566 | 2.1E + 08 | 321 | 1.2E + 08 |
5 | 15 | 1449 | 5.3E + 08 | 849 | 3.2E + 08 | 482 | 1.8E + 08 |
6 | 20 | 1931 | 7.1E + 08 | 1132 | 4.3E + 08 | 643 | 2.4E + 08 |
7 | 50 | 4829 | 1.8E + 09 | 2831 | 1.1E + 09 | 1607 | 5.9E + 08 |
8 | 100 | 9657 | 3.5E + 09 | 5661 | 2.1E + 09 | 3213 | 1.2E + 09 |
Spike (1 ng) contains | 3.7E + 08 |  | 3.8E + 08 |  | 3.7E + 08 |