Fig. 4

Evenness of contig coverage in saliva viromes obtained by SISPA. a Two representative cross-contigs with high-coverage peaks surrounded by sequences with similarity to the constant part of the primers (coloured triangles) used during SISPA. b Four representative cross-contigs from SISPA viromes with high-coverage peaks in regions with low linguistic sequence complexity. c Coefficients of coverage variation for the 14 most abundant contigs sharing > 50× average coverage among analysed viromes. d Pearson’s correlations among cross-contig coverage profiles of unamplified and de-multiplexed SISPA-amplified viromes; *p < 0.01; **p < 0.005; and ***p < 0.001