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Table 1 Overview of 19 sequencing runs with bacterial mock positive controls, repeat stool samples, and negative controls over a 2.5-year time period. All samples were processed in the same manner and run on the same sequencing machine. Run 18 includes a prospective dilution study of the mock bacterial controls. Numbers in parenthesis include the samples initially in analysis before negative control filtering

From: Quantification of variation and the impact of biomass in targeted 16S rRNA gene sequencing studies

Sample type

Run 1

Run 2

Run 3

Run 4

Run 5

Run 6

Run 7

Run 8

Run 9

Run 10

Run 11

Run 12

Run 13

Run 14

Run 15

Run 16

Run 17

Run 18

Run 19

Total

Bacterial mock

  

5

7

2

19

4

3

3

2

2

3

12

1

13

4

14

105 (110)

14

213 (218)

Stool

8

15

6

                

29

Negatives

 

(5)

(7)

(12)

 

(1)

(3)

(7)

(5)

(6)

(7)

(11)

(38)

(10)

(33)

(10)

1(27)

(13)

1 (27)

2(222)

Analysis

                    

Mocks over time

  

●

●

●

●

●

●

●

●

●

●

●

^

●

●

●

*

●

117

Biological vs technical variation

●

●

●

●

●

●

●

●

●

●

●

●

●

^

●

●

●

*

●

146

Biomass

                 

●

 

105

  1. ●Included in the analysis
  2. ^Excluded due to only having a single mock sample in this run
  3. *Only includes 10 undiluted mock samples from this run
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Microbiome

ISSN: 2049-2618

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