From: Quantification of variation and the impact of biomass in targeted 16S rRNA gene sequencing studies
a. Model | Estimate | Standard error | t value | p value | Â | Â |
(Intercept) | 1.43238 | 0.12556 | 11.408 | < 2.2E−16 |  |  |
log10 copies/microliter | − 0.2816 | 0.04905 | − 5.741 | 6.93E−8 |  |  |
log10 mean relative abundance | − 0.54936 | 0.06927 | − 7.931 | 1.13E−12 |  |  |
Residual standard error | 0.5196 | Â | Â | Â | Â | Â |
Multiple R-squared | 0.4496 | Â | Â | Â | Â | Â |
Adjusted R-squared | 0.4407 | Â | Â | Â | Â | Â |
F statistic | 50.25 on DF (2123) | Â | Â | Â | Â | |
b. Prediction | Â | Mean relative abundance (%) | ||||
 | Copies/microliter | 1 | 5 | 10 | 25 | 50 |
Low biomass | 10 | 0.5723 | 1.7313 | 2.7888 | 5.2376 | 8.4370 |
Medium biomass | 1000 | 0.2865 | 0.8668 | 1.3963 | 2.6224 | 4.2242 |
High biomass | 100,000 | 0.1435 | 0.4340 | 0.6991 | 1.3130 | 2.1150 |