Table 2 PCR primers targeting genes coding for antibiotic resistance
From: Longitudinal development of the dust microbiome in a newly opened Norwegian kindergarten
Gene | Primer sequence | PCR conditions | Approximate amplicon size (bp) |
|---|---|---|---|
mecA | 5′-GTAGAAATGACTGAACGTCCGATAA-3′ 5′-CCAATTCCACATTGTTTCGGTCTAA-3′ [51] | 4 min 94 °C;30 cycles (45 s at 94 °C; 45 s at 50 °C; 60 s at 72 °C); 2 min at 72 °C | 310 |
ermA | 5′-GTTCAAGAACAATCAATACAGAG-3′ 5′-GGATCAGGAAAAGGACATTTTAC-3′ [52] | 5 min 94 °C; 30 cycles (30 s at 94 °C; 30 s at 52 °C, 60 s at 72 °C); 10 min 72 °C | 421 |
aac(6′)-aph(2″) | 5′-TTGGGAAGATGAAGTTTTTAGA-3′ 5′-CCTTTACTCCAATAATTTGGCT-3′ [53] | 3 min 95 °C; 35 cycles (20 s at 95 °C; 20 s at 57 °C; 30 s at 72 °C); 2 min 72 °C | 174 |
vanA | 5′-CATGAATAGAATAAAAGTTGCAATA-3′ 5′-CCCCTTTAACGCTAATACGATCAA-3′ [54] | 10 min 95 °C; 30 cycles (30 s at 94 °C; 30 s at 58 °C; 30 s at 72 °C); 10 min 72 °C | 1030 |