Fig. 4

Distribution of Chloroflexi-reconstructed MAGs in freshwater and brackish environments. The recruitment (RPKG) distribution of reconstructed MAGs of Chloroflexi cluster SL56 (a), TK10 (b), and CL500-11 (c) against freshwater and brackish datasets. Freshwater datasets belong to the lakes and reservoirs from Europe (16), Asia (9), South (5), and North America (47) and brackish datasets include three depths (15 m, 40 m, and 150 m) datasets of the Caspian Sea (complete list of datasets used, and their metadata is available in Additional file 4: Table S3). The hypolimnion datasets of Lake Zurich, Lake Biwa, and Caspian Sea are shown in black boxes. Genomes belonging to the same species are shown in a gray box