Fig. 2

Bacteroides dorei is lysogenized by a DGR-containing temperate phage. a Coverage plot of viral reads aligning to a 66 kb region in Bacteroides dorei predicted to contain a prophage. b ORFs encoded by the discrete 43 kb region with high read coverage are represented by arrows and colored according to function. Red, enzymatic; green, structural; gray, hypothetical. The DGR components are indicated as RT, reverse transcriptase; TR, template repeat; VR, variable repeat. c Transmission electron microscopy image of a phage lysate prepared from an induced culture of B. dorei (scale bar = 100 nm). d Agarose gel showing PCR products using primers indicated on b and universal 16S rRNA gene primers. The upper panel depicts PCR products using B. dorei DNA as a template and the lower panel depicts products from the purified phage lysate. All primer sequences are listed in Additional file 13. For both panels, the four lanes on the right are no-template controls