Table 1 Summary of the primer characteristics and specificity
Taxon targeted | Amplicon length (in bp) | No. species amplifiable (%NCBI*) | Matching single genus (mean) | Matching single species (mean) | Specificity (%on target) | Forward primer | Reverse primer |
|---|---|---|---|---|---|---|---|
Amoebozoa | 333–420 | 265 (69.9%) | 98.7% (1) | 83.7% (1.3) | 47.4% | GAATTGACGGAAGGGCACAC | GCCCYRTCTAAGGGCATCAC |
Apicomplexa A | 244–248 | 434 (80.4%) | 84.8% (1.3) | 55.9% (6.8) | 99.1% | GACCTATCAGCTTTCGACGG | CCCTCCAATTGWTACTCTGGR |
Apicomplexa B | 228–244 | 95.6% (1.2) | 73.1% (3.4) | 94.1% | TGYGTTTGAATACTAYAGCATGG | TCTGATCGTCTTCACTCCCTT | |
Apicomplexa C | 420–470 | 99.1% (1) | 76.2% (1.4) | 83.1% | TTGGMCTACCGTGGCARTGA | TCAAGGCAAHWGCCTGCTT | |
Blastocystis | 303–307 | 4 (66.7%) | 100% (1) | 94.1% (1.1) | 80.0% | TGGTCGCAAGGCTGAAACTT | TTGCCTCCAGCTTCCCTACA |
Diplomonadida | 277–285 | 15 (83.3%) | 100% (1) | 93.8% (1.1) | 100.0% | RGGGACRGGTGAAATAGGATG | CAAATTGAGCCGCAGACTCC |
Kinetoplastida | 250–300 | 188 (95.9%) | 92.2% (1.1) | 83.2% (1.5) | 96.7% | AAATTAAACCGCACGCTCCA | GCAAACGATGACACCCATGA |
Microsporidia | 370–440 | 96 (60.4%) | 96.5% (1) | 92.5% (1.2) | 100.0% | BCAGGTTGATTCTGCCTGACR | ACCAGWCTTGCCCTCCARTT |
Parabasalia | 326–364 | 105 (85.4%) | 100% (1) | 96.8% (1) | 100.0% | TAGGCTATCACGGGTAACGG | GCGTCCTGATTTGTTCACAG |
Platyhelminthes | 350–550 | 778 (56.2%) | 96.6% (1.1) | 88.7% (1.3) | 30.3% | CAATTGGAGGGCAAGTCTGG | TGCTTTCGCWKTAGTTTGTCTG |
Nematode A | 320–335 | 1233 (70%) | 87.7% (1.3) | 68.1% (2) | 99.2% | CACCCGTGAGGATTGACAG | CGATCACGGAGGATTTTCAA |
Nematode B | 380–410 | 96.4% (1.1) | 82.2% (1.3) | 99.9% | CGTCATTGCTGCGGTTAAAA | CCGTCCTTCGAACCTCTGAC | |
Nematode C | 380–440 | 92.2% (1.1) | 74.4% (1.7) | 62.5% | AGTGGAGCATGCGGCTTAAT | TGCAATTCCCTRTCCCAGTC |