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Table 2 Number of differentially expressed (DE) genes per KEGG category of selected MAGs in R1 (single-stage configuration) and R3 (methanogenic reactor of the two-stage configuration)

From: Microbial activity response to hydrogen injection in thermophilic anaerobic digesters revealed by genome-centric metatranscriptomics

MAG

M. thermophila UC0006

M. wolfeii UC0008

C. proteolyticus UC0011

T. acetatoxydans UC0018

A. hydrogeniformans UC0046

D. tunisiensis UC0050

 

Reactor

KEGG category

R3

R1

R3

R1

R3

R1

R3

R1

R1

R3

ABC transporters

3

5

4

2

0

7

10

4

0

11

Amino acids metabolism

19

5

0

0

5

5

35

8

0

21

Bacterial chemotaxis

0

0

0

0

0

3

8

1

0

5

Biosynthesis of amino acids

43

4

3

0

4

3

37

2

0

39

Biosynthesis of antibiotics

18

8

4

3

10

4

28

1

0

6

Biosynthesis of secondary metabolites

13

8

3

3

5

4

28

0

0

20

Butanoate metabolism

3

1

1

1

6

0

3

0

0

7

Carbon fixation pathways in prokaryotes

3

4

2

0

7

0

5

1

0

5

Carbon metabolism

18

21

12

4

11

4

19

1

0

11

Citrate cycle (TCA cycle)

1

4

1

2

9

0

6

0

0

5

Fatty acid metabolism

0

0

0

0

0

0

0

2

0

1

Flagellar assembly

0

0

0

0

0

0

7

0

0

0

Galactose metabolism

1

0

0

2

0

5

6

0

0

1

Glycolysis/Gluconeogenesis

1

1

1

2

7

4

11

0

0

5

Metal transport system

6

0

5

0

1

0

6

0

0

8

Methane metabolism

21

64

14

0

0

0

7

0

0

3

Nitrogen metabolism

5

0

0

0

1

1

3

1

0

1

Peptidoglycan biosynthesis

0

0

0

0

1

0

3

0

0

2

Propanoate metabolism

1

0

1

1

1

1

2

2

0

1

Purine metabolism

0

4

0

1

0

1

0

0

0

0

Pyrimidine metabolism

6

5

1

1

3

0

0

0

0

1

Pyruvate metabolism

0

3

0

3

0

1

0

0

0

0

Quorum sensing

4

0

0

1

2

0

13

0

3

7

Reductive acetyl-CoA pathway (Wood-Ljungdahl)

1

1

0

0

0

2

0

0

0

0

Ribosome

28

25

5

3

1

0

16

0

0

5

Sugar, amino acid and oligo-peptide transport system

0

0

0

0

1

0

25

0

0

1

Triacylglycerol biosynthesis

1

0

0

0

0

0

1

0

0

2

Two-component system

2

0

0

0

0

0

9

0

0

5