Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data

Microbiome

Table 1 Amplicon sequence variants from placenta samples classified by decontam as non-contaminants

	P (adjusted)	Taxa	Placenta (n = 24)	Vagina (n = 6)
1	1.2 × 10⁻⁵	Homo sapiens	19	1
2	0.11	Homo sapiens	11	0
3	0.17	Homo sapiens	9	0
4	0.17	Homo sapiens	9	0
5	0.47	Ruminococcaceae sp.	8	0
6	0.47	Homo sapiens	8	1

Eight hundred ten ASVs present in at least five samples were evaluated by the prevalence method as implemented in the isNotContaminant function. The six ASVs classified as non-contaminants using an FDR threshold of 0.5 [56] are shown. Taxonomy was assigned by BLAST-ing sequences against the nt database. Prevalence is reported for each sample type included in the study

ISSN: 2049-2618