Fig. 1
From: Design and application of a novel two-amplicon approach for defining eukaryotic microbiota
Identification and evaluation of biomarker regions in 18S and 28S rRNA genes. a Sequence variability of protozoan 18S and 28S rRNA genes. Variability was calculated as the Shannon entropy at each position along aligned protozoan sequences. Identified variable regions are numbered and those selected for further analysis are highlighted in purple. The graphic at the top depicts the canonical arrangement of eukaryotic rRNA genes. b Accuracy of taxonomic classification of variable regions to the phylum, genus, and species levels. Taxonomies of 100 copies of variable regions in each gene, randomly mutated with 1% frequency, were compared to the best match identified by BWA-MEM in the SILVA v128 database. Results are shown for protozoan (19,427 18S; 1061 28S), helminth (3882 18S; 1237 28S), and fungal (14,657 18S; 3625 28S) rRNA genes