Skip to main content
Fig. 6 | Microbiome

Fig. 6

From: Metagenomic, phylogenetic, and functional characterization of predominant endolithic green sulfur bacteria in the coral Isopora palifera

Fig. 6

Nitrogen fixation activity of the endolithic culture from the green layer of the coral skeleton. a Acetylene reduction assay as proxy for nitrogen fixation activity in endolithic culture N2. Basal medium was used as control and sterilized N2 as negative control. a, b Indicate significantly difference in the concentration of C2H4 (p < 0.002) production for each time point by t-test between N2 and controls. b Parallel FISH-NanoSIMS images of endolithic enrichment culture (N2) before (b, c, d) and after (e, f, g) 15N2 incubation. The FISH results before (b) and after (e) 15N2 incubation show the endolithic GSB (green) and other microbes (red). Natural abundance of nitrogen isotopic composition (12C15N/12C14N) is 0.00367 and is black in the color bar in both images c and f. The 32S images shows the distribution of all microorganisms before d and after g 15N2 incubation. Scale bars indicate 5 μm

Back to article page