Skip to main content

Table 3 Collection media type and room temperature incubation times evaluated in the study

From: Collection media and delayed freezing effects on microbial composition of human stool

Media tested

Incubation time 0

Incubation time 1

Incubation time 2

(72 h at 25 °C)

(168 h at 25 °C)

No media

3 Aliquots

–

–

RNAlater®

3 Aliquots

3 Aliquots

3 Aliquots

RNAlater® + Kana

3 Aliquots

3 Aliquots

3 Aliquots

RNAlater® + Ciprob

3 Aliquots

3 Aliquots

3 Aliquots

  1. Study participants (n = 10) sampled aliquots (n = 30) from a single stool which they stored under different conditions. Subsamples were collected either without collection media or three different RNAlater-based media and frozen immediately after collection (time 0) or stored by two time periods (72 and 168 h) at 25 °C before freezing on dry ice and stored at −80 °C
  2. aRNAlater® supplemented with 300 μg/ml Kanamycin
  3. bRNAlater® supplemented with 300 μg/ml Ciprofloxacin
Back to article page

Microbiome

ISSN: 2049-2618

Contact us