Fig. 7

ALA-FMT mitigated colitis induced by T. gondii cyst infection. A Diagram of FMT experiments. B Mouse weight loss. C Mouse survival rate (n = 6 per group). Statistical significance was determined using two-way ANOVA. ***p < 0.001, **p < 0.01, *p < 0.05 compared with the PBS-FMT group. The survival rate was determined using the log-rank (Mantel-Cox) test. **p < 0.01 compared with the PBS-FMT group. D–E H&E staining of the mouse (n = 5 per group) colon tissues (scale bars = 200 μm, × 10 objective). Representative images are shown. F–I The levels of IFN-γ, TNF-α, IL-1β, and IL-6 were measured by ELISA (n = 5 per group). Statistical significance was determined with Student’s t test. **p < 0.01, *p < 0.05. J The expression level of MyD88 was measured by immunofluorescence. MyD88 protein was labelled with a red fluorophore, and the cell nucleus was labelled with a blue fluorophore (Scale bars = 200 μm, × 10 objective). Representative images are shown. K–M The expression levels of p-NF-κB p65 and occludin were measured by Western blotting. β-actin was used as a control. The data are presented as the means ± SEM. Statistical significance was determined with Student’s t test. *p < 0.05. All experimental data are representative of three independent experiments with similar results