Table 1 Summary table of all 16S rRNA gene and metabolomic data generated
From: Decline of a distinct coral reef holobiont community under ocean acidification
|  | Metabarcoding — 16S rRNA gene | Metabolomics — MeOH solvent extraction | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Community fractions | Single holobiont fractions | All 16s rRNA gene | Community fractions | Single holobiont fractions | All metabolomes | |||||
Sediment | Benthic photosynthetic community | Benthic holobiont community | Sponge | Sediment | Benthic photosynthetic community | Benthic holobiont community | Sponge | |||
Samples (control, medium, low) | 17 (6; 5; 6) | 15 (5; 5; 5) | 15 (6; 4; 5) | 47 (18; 13; 16) | 94 (35; 27; 32) | 18 (6; 6; 6) | 19 (7; 6; 6) | 18 (6; 6; 6) | 52 (19; 16; 17) | 107 (38; 34; 35) |
Median ASVs/compounds per sample (mean) | 11,553 (11,437) | 2497 (2575) | 7414 (7558) | 1756 (1902) | 2596 (4685) | 192.5 (191) | 176 (169) | 164 (172) | 172 (181) | 178 (179) |
Median reads/biomolecules per sample (mean) | 226,704 (223,047) | 191,380 (189,806) | 228,828 (231,210) | 179,696 (188,960) | 199,515 (202,050) | 217,272,202 (222,010,376) | 164,003,665 (184,941,718) | 149,861,437 (163,084,039) | 200,557,415 (208,791,822) | 189,723,756 (199,091,279) |
ASVs identified at phylum/compounds identified (percent reads/molecules) | 70.8 (87.8) | 76.9 (87.4) | 74.1 (73.4) | 74.9 (71.9) | 69 (77.7) | 5.04 (4.27) | 4.77 (1.57) | 6 (3.29) | 7.79 (8.8) | 4.62 (6) |
Median phyla per sample (mean) | 57 (56.6) | 29 (30.5) | 46 (45.9) | 38 (37.8) | 40 (41.4) | NA | NA | NA | NA | NA |
Median ASV/metabolomic richness per sample (mean) | 11,985 (11,920) | 2750 (2802) | 7909 (8006) | 1984 (2098) | 2991 (5071) | 192 (191) | 176 (169) | 164 (172) | 172 (181) | 178 (179) |
Median ASV/metabolomic Shannon diversity per sample (mean) | 2.12 (2.13) | 1.4 (1.39) | 2.02 (2.02) | 0.971 (0.92) | 1.16 (1.39) | 2.83 (2.81) | 2.99 (2.83) | 2.76 (2.76) | 2.96 (3.04) | 2.87 (2.92) |