Fig. 2

Fungal reconstruction maintains diet-induced obesity. A–C Body weight gain (A), the relative weight of white adipose tissues, and adipocyte size and area of SAT and PEAT (HE staining, × 400) in flu-pretreated mice. C57BL/6 mice (6 weeks old, male) received 1-week fluconazole (PreFlu) and then fed HFD (PreFlu + HFD) lasted for 20 weeks (n = 10). D–G Body weight gain (D), the relative weight of SAT (E), adipocyte area of SAT (HE staining, × 400), and serum bile acid (TBA) (G) in flu-pretreated mice cohousing with control mice. C57BL/6 mice (7 weeks old, male) with PreFlu and HFD exposure were cohoused with (PreFlu + HFD + cohouse) or without healthy mice for 14 weeks to rebuilt gut fungal communities (n = 10). H–K Body weight (H), the relative weight of white adipose tissues (I), adipocyte area of PEAT (HE staining, × 400), and serum glucose (K) in Flu-pretreated mice cohousing with healthy or obese mice. C57BL/6 mice (7 weeks old, male) with PreFlu and HFD exposure were cohoused with obese mice (31.67 ± 0.37 g) for 18 weeks to rebuilt gut fungal communities (n = 7–10). L–N Body weight gain (L), the relative weight of white adipose tissues (M), and adipocyte area of SAT and PEAT (HE staining, × 400) in Flu-pretreated mice with fecal microbiota transplantation (FMT). C57BL/6 mice (7 weeks old, male) with PreFlu and HFD exposure were transplanted with fecal microbiota from healthy donors (PreFlu + HFD (FMT)) for 16 weeks to rebuilt gut fungal communities (n = 4–10). Differences among the groups were compared using Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001; ns p > 0.05