Fig. 6

Comparative analyses among culturable fungi isolated by dilution-plate method (DPM), fungal isolation chips (FiChips), and high-throughput sequencing (HTS) from SZ-1 and SZ-2 sediment samples. a The heatmap for generic diversity from two sediment samples. The label ALL indicates the corresponding total generic diversity of the isolates. b Venn diagram of species between DPM and FiChips from two sediment samples. c Novelty of fungal strains grown in FiChips and DPM. The x-axis shows the sequence novelty, in percentage of diversion from the known species. The y-axis shows the number of species cultivated via each indicated method. Fungal strains were more novel as compared with strains cultivated using standard dilution-plate methods. d The interactive network of ITS2 locus sequenced from DPM, FiChips, and HTS. The nodes indicate different ITS2 genes. The nodes in green, pink, and blue indicate the ITS2 locus sequenced from HTS, FiChips, and DPM, respectively. The nodes in purple indicate the ITS2 locus sequenced from any two methods. Candidate novel species are indicated by different shape of nodes (square nodes indicate ITS sequence similarity between 94 and 98% comparing to the NCBI database, triangular nodes indicate similarity between 91 and 94%, and rhombic nodes indicate similarity below 91%). e The composition of species isolated from DPM and FiChips based on the criteria of abundant taxa, intermediate taxa, and rare taxa