Fig. 7

Blockade of interaction between DVF and LRRC19 by typhaneoside alleviates the pro-inflammatory effect of DVF in mice. A Molecular docking results of high-throughput screening based on the structure of DVF/LRRC19 complex. B Predicted binding modes of TYP and DVF/LRRC19 complex and its three-dimensional structure. C Biacore analysis of the interaction between typhaneoside and LRRC19. D Microscale thermophoresis result for the binding of DVF to LRRC19 in the presence of TYP. E The experimental design of DSS model. F Body weight was presented as a percentage of the initial weight. G–J DAI (G), representative images of anal bleeding (H), colon length (I), and spleen weight (J) are shown. K Representative histological images of colon tissues by H&E staining (left panel) and histopathological score (right panel). Scale bars, 500 µm. L IHC staining and quantitation of LRRC19 in the colonic mucosa of colitis mice. Scale bars, 50 µm. M The relative mRNA expression of Cxcl9, Cxcl10, IL1β, and Tnf-α in colon tissues. All data are presented as mean ± SEM. *P < 0.05, *** P < 0.001, ns, not significant. Two-tailed Student’s t test in F, G; one-way ANOVA in I–L; two-tailed Mann–Whitney U test in M