Fig. 3
Pathway and carbon utilization pattern of ANME-1 and Bathy-8 archaea. Gene presence (a) and pathways involved in carbon utilization by ANME-1 and Bathy-8 with carbon contribution from organic carbon added besides the compounds (b). Black circle, presence of gene in MAG; white circle, absence of gene in MAG. Green arrows indicate the AOM pathway in ANME-1 and orange arrow indicates the putative transformation of methoxy groups by Bathy-8. Black arrows indicate the pathways for both ANME-1 and Bathy-8. Dashed arrows denote reactions catalyzed by multiple enzymes. The contribution of organic carbon to nucleic acids was based on the previous study of methylotrophic methanogens [56]. rTCA, reductive tricarboxylic acid cycle; RGP, reductive glycine pathway; RHP, reductive hexulose-phosphate pathway; WL, Wood–Ljungdahl pathway; CH2=H4MPT, methylene-tetrahydromethanopterin; CH3-H4MPT, methyl-tetrahydromethanopterin; CH3-CoM, methyl-coenzyme M